Isolation of Mammalian Cells and Colonization of Implant Blanks
Zellwerk produces cell formulations or colonized implant blanks for clinical and research use according to your specifications. Depending on the customers requirements all procedures are either conducted under stringent quality control measures following cGMP- and cGCP-standards or in less costly experimental environments. Culturing in rotating bed bioreactors has proven as superior way to generate large amounts of primary mammalian (human) cells. Up to 1010 viable cells can be isolated from long-term cultivation runs in a Z® RP Bioreactor.
Sponceram® discs after long-term cultivation of a human liver cell line in Z® RP Bioreactor, density coated with a layer of cells and ECM.
Zellwerk´s client service comprises extraction of primary cells from tissue samples, cell type selection, expansion, harvesting and transfer into a stable cell formulation. Validated protocols for enzymatic extraction, characterisation, cultivation and isolation of several cell types were developed.

The following examples, performed at Zellwerk´s labs, are of clinical interest: Tissue-like grown human hepatocytes can serve as a model for an artificial liver (see images). Human ceratinocytes, forming skin-like multilayer structures when colonized on our spiral-shaped rotating bed (see products), offer an advantageous way to produce larger human skingrafts .
Electron-microscopic capture of ECM with embedded liver cells, cultivated on a Sponceram®; disc; magnification 1: 500.
Hiper AG